FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005466441

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005466441
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8342162
Sequences flagged as poor quality0
Sequence length150
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGTGTCTGGGGAGCACTGTGTGTTCACGGCACAGAGGTAGGTGGCTGAA143440.17194583370593858No Hit
ACTGTGTGTTCACGGCACAGAGGTAGGTGGCTGAATCACTGGGCTGGGAG133530.160066419232808No Hit
GTTCACGGCACAGAGGTAGGTGGCTGAATCACTGGGCTGGGAGTCTCTGA133430.15994654623106097No Hit
GGCACAGAGGTAGGTGGCTGAATCACTGGGCTGGGAGTCTCTGATGAGCA132360.15866390511236775No Hit
CACGGCACAGAGGTAGGTGGCTGAATCACTGGGCTGGGAGTCTCTGATGA122270.14656871923609252No Hit
TGTGTGTTCACGGCACAGAGGTAGGTGGCTGAATCACTGGGCTGGGAGTC117850.14127033255887383No Hit
CACTGTGTGTTCACGGCACAGAGGTAGGTGGCTGAATCACTGGGCTGGGA115770.13877697412253562No Hit
CAGGTGTCTGGGGAGCACTGTGTGTTCACGGCACAGAGGTAGGTGGCTGA110030.13189626382225617No Hit
AGCACTGTGTGTTCACGGCACAGAGGTAGGTGGCTGAATCACTGGGCTGG110030.13189626382225617No Hit
CTGTGTGTTCACGGCACAGAGGTAGGTGGCTGAATCACTGGGCTGGGAGT106670.12786853096355597No Hit
TGTTCACGGCACAGAGGTAGGTGGCTGAATCACTGGGCTGGGAGTCTCTG102800.12322944579594595No Hit
CTGGGGAGCACTGTGTGTTCACGGCACAGAGGTAGGTGGCTGAATCACTG102210.12252219508563848No Hit
CTGCAGGTGTCTGGGGAGCACTGTGTGTTCACGGCACAGAGGTAGGTGGC101030.12110769366502352No Hit
TGTGTTCACGGCACAGAGGTAGGTGGCTGAATCACTGGGCTGGGAGTCTC99240.11896196693375172No Hit
ACTGCAGGTGTCTGGGGAGCACTGTGTGTTCACGGCACAGAGGTAGGTGG97750.11717585920772097No Hit
CAAGTGTCTGGGGAGCACTGTGCGCTCATTGCACAGAGGTAGGTGGCTGA94430.11319607554971961No Hit
GCACTGTGTGTTCACGGCACAGAGGTAGGTGGCTGAATCACTGGGCTGGG93310.11185349793015288No Hit
TGTCTGGGGAGCACTGTGTGTTCACGGCACAGAGGTAGGTGGCTGAATCA92730.11115823452002012No Hit
ATTGCACAGAGGTAGGTGGCTGAATCACTGGGCTGTGAGTCTCTGATGAA92480.11085855201565253No Hit
GCTCATTGCACAGAGGTAGGTGGCTGAATCACTGGGCTGTGAGTCTCTGA91170.10928821569276645No Hit
GTGTGTTCACGGCACAGAGGTAGGTGGCTGAATCACTGGGCTGGGAGTCT90410.10837718087948903No Hit
CATTGCACAGAGGTAGGTGGCTGAATCACTGGGCTGTGAGTCTCTGATGA90130.10804153647459735No Hit
TTCACGGCACAGAGGTAGGTGGCTGAATCACTGGGCTGGGAGTCTCTGAT89690.10751409526691043No Hit
CAATAAAGCCAGCCAGTATGTTTCTCTGCTCATCAGAGACTCCCAGCCCA89100.10680684455660296No Hit
GGAGCACTGTGTGTTCACGGCACAGAGGTAGGTGGCTGAATCACTGGGCT87890.10535638123546391No Hit
ACTGTGCGCTCATTGCACAGAGGTAGGTGGCTGAATCACTGGGCTGTGAG87760.10520054633319277No Hit
GAGCACTGTGTGTTCACGGCACAGAGGTAGGTGGCTGAATCACTGGGCTG87520.1049128511289999No Hit
GTCTGGGGAGCACTGTGTGTTCACGGCACAGAGGTAGGTGGCTGAATCAC85520.10251539109405931No Hit
GGGAGCACTGTGTGTTCACGGCACAGAGGTAGGTGGCTGAATCACTGGGC83740.10038165166296219No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGCGA701.769221E-551.4225928
TCTCGCG605.1220035E-447.9941337
TCGCGAG800.002108941835.9962469
TAGTCGA1358.422593E-426.6659665
ACGCCAA2551.0164695E-725.4110983
TTCGCAG2301.1746051E-625.040578
CGTTTCG1450.001278488124.8249999
CTTATCG2151.9724615E-523.4389957
GCTACGC2202.3473633E-522.9174181
CGCACTA1902.3007546E-422.7362443
CCGTCCC4205.820766E-1122.2851284
ATTTCGC4609.094947E-1221.9124666
GTACGCA1650.002711046721.8155167
TCGCGAA403.2560682E-421.59865660-64
TTTCGCA2353.920647E-521.444197
ACGTACC2053.845796E-421.0726175
CGTACCA2104.5249742E-420.5708876
GTCCGGA2104.5279026E-420.5689167
GTTCACC25950.019.1445792
GGCGAGT1900.00613261718.9468695
Produced by FastQC (version 0.11.5)