FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005466476

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005466476
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2488326
Sequences flagged as poor quality0
Sequence length150
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATACTCCAATGGTGACAAAGAAGATGGAAGGTTTACAGCACAGCTCAAT131050.5266592882122358No Hit
CACAGCTCAATAAAGCCAGCCAGTATGTTTCTCTGCTCATCAGAGACTCC121010.4863108772725117No Hit
GTAACAAAGAAGATGGAAGGTTTACAGCACAGGTCGATAAATCCAGCAAG115500.4641674764480217No Hit
AGCACTGTGTGTTCACGGCACAGAGGTAGGTGGCTGAATCACTGGGCTGG110900.44568115271069786No Hit
ACAGCACAGCTCAATAAAGCCAGCCAGTATGTTTCTCTGCTCATCAGAGA109770.44113994709696397No Hit
TGCACAGAGGTAGGTGGCTGAATCACTGGGCTGTGAGTCTCTGATGAACA107450.43181640990770503No Hit
ATTGCACAGAGGTAGGTGGCTGAATCACTGGGCTGTGAGTCTCTGATGAA102180.41063751293037964No Hit
TGTTGGGAAAAGCCCTGAGTTGATAATGTTCATATACTCCAATGGTGACA100970.40577480603425753No Hit
GCAGGTGTCTGGGGAGCACTGTGTGTTCACGGCACAGAGGTAGGTGGCTG98690.3966120194861927No Hit
TGGGGAGCACTGTGTGTTCACGGCACAGAGGTAGGTGGCTGAATCACTGG98420.3955269526581324No Hit
GATGGAAGGTTTACAGCACAGCTCAATAAAGCCAGCCAGTATGTTTCTCT96090.3861632278085749No Hit
CTGTGTGTTCACGGCACAGAGGTAGGTGGCTGAATCACTGGGCTGGGAGT95650.3843949707554396No Hit
CACTGTGTGTTCACGGCACAGAGGTAGGTGGCTGAATCACTGGGCTGGGA94770.38085845664916895No Hit
GCACTGTGTGTTCACGGCACAGAGGTAGGTGGCTGAATCACTGGGCTGGG93400.37535274718827033No Hit
CAGGTGTCTGGGGAGCACTGTGTGTTCACGGCACAGAGGTAGGTGGCTGA92460.3715751071202085No Hit
TCCATATACTCCAATGGTGACAAAGAAGATGGAAGGTTTACAGCACAGCT91890.3692844104831923No Hit
TCACGGCACAGAGGTAGGTGGCTGAATCACTGGGCTGGGAGTCTCTGATG91840.3690834721816997No Hit
AATAAAGCCAGCCAGTATGTTTCTCTGCTTATCAGAGACTCCCAGCCCAG85950.3454129402658655No Hit
GAAGGTTTACAGCACAGCTCAATAAAGCCAGCCAGTATGTTTCTCTGCTC81400.3271275548300343No Hit
TGTCCATATACTCCAATGGTGACAAAGAAGATGGAAGGTTTACAGCACAG79350.3188890844688357No Hit
GGCACAGAGGTAGGTGGCTGAATCACTGGGCTGGGAGTCTCTGATGAGCA78030.3135843133094297No Hit
CAGTATTCCAGAAAAGGCCCTGAGTTGCTGATGTACACATACTCCAGTGG77940.3132226243667429No Hit
GGAAAAGCCCTGAGTTGATAATGTCCATATACTCCAATGGTGACAAAGAA77510.31149455497390616No Hit
GGAGCACTGTGTGTTCACGGCACAGAGGTAGGTGGCTGAATCACTGGGCT73940.29714756024733097No Hit
TGTGCGCTCATTGCACAGAGGTAGGTGGCTGAATCACTGGGCTGTGAGTC71810.28858758860374406No Hit
TGTCTGGGGAGCACTGTGTGTTCACGGCACAGAGGTAGGTGGCTGAATCA71560.2875828970962808No Hit
AAGTGTCTGGGGAGCACTGTGCGCTCATTGCACAGAGGTAGGTGGCTGAA70900.28493051151657783No Hit
ACTGTGTGTTCACGGCACAGAGGTAGGTGGCTGAATCACTGGGCTGGGAG69290.2784602982085145No Hit
TGTGTTCACGGCACAGAGGTAGGTGGCTGAATCACTGGGCTGGGAGTCTC69290.2784602982085145No Hit
TCCTTCTTCTGGTACAGACAATATTCTGGGAAAAGCCCTGAGTTGATAAT69080.2776163573422453No Hit
TGATAATGTCCATATACTCCAATGGTGACAAAGAAGATGGAAGGTTTACA67750.27227139852254084No Hit
CAGAGGGAGCCATTGTTTCTCTCAACTGCACTTACAGCAACAGTGCTTTT63720.25607577142223326No Hit
TTGCACAGAGGTAGGTGGCTGAATCACTGGGCTGTGAGTCTCTGATGAAC61990.24912330619058756No Hit
CAATGGTGACAAAGAAGATGGAAGGTTTACAGCACAGCTCAATAAAGCCA59580.23943808005864187No Hit
TATGTTTCTCTGCTCATCAGAGACTCCCAGCCCAGTGATTCAGCCACCTA57340.2304360441517711No Hit
GTGTCTGGGGAGCACTGTGTGTTCACGGCACAGAGGTAGGTGGCTGAATC57240.2300341675487858No Hit
CATTCATGTGGTACAGACAGTATTCCAGAAAAGGCCCTGAGTTGCTGATG55840.2244078951069916No Hit
CCAGTATGTTTCTCTGCTCATCAGAGACTCCCAGCCCAGTGATTCAGCCA55560.2232826406186328No Hit
TGCAGGTGTCTGGGGAGCACTGTGTGTTCACGGCACAGAGGTAGGTGGCT55430.2227602010347519No Hit
AGTCTTCTTCTGGTACAGACAATATTCTGGGAAAAGCCCTGAGTTGATAA55220.22191626016848276No Hit
AGGTGTCTGGGGAGCACTGTGTGTTCACGGCACAGAGGTAGGTGGCTGAA55200.2218358848478857No Hit
CTTCAAAGAAGATGGAAGGTTTACAGCACAGCTCAATAAAGCCAGCCAGT54940.22079100568012391No Hit
GATCGGCACAGAGGTAGGTGGCTGAATCACTGGGCTGGGAGTCTCTGATG53830.2163301753869871No Hit
GTCCATATACTCCAATGGTGACAAAGAAGATGGAAGGTTTACAGCACAGC53490.21496379493683707No Hit
CCATATACTCCAATGGTGACAAAGAAGATGGAAGGTTTACAGCACAGCTC52400.2105833399642973No Hit
CAAAGAAGATGGAAGGTTTACAGCACAGCTCAATAAAGCCAGCCAGTATG52230.20990014973922225No Hit
ATGGAAGGTTTACAGCACAGCTCAATAAAGCCAGCCAGTATGTTTCTCTG51900.20857395694937078No Hit
CCATTGCCTCTCTCAACTGCACTTACAGTGACCGAGGTTCCCAGTCCTTC51210.20580100838877222No Hit
GATAATGTCCATATACTCCAATGGTGACAAAGAAGATGGAAGGTTTACAG50550.20314862280906926No Hit
AGAGACTCCCAGCCCAGTGATTCAGCCACCTACCTCTGTGCCGTGAACAC49800.20013454828667945No Hit
TTCACGGCACAGAGGTAGGTGGCTGAATCACTGGGCTGGGAGTCTCTGAT47660.19153438898279407No Hit
GCACAGCTCAATAAAGCCAGCCAGTATGTTTCTCTGCTCATCAGAGACTC47410.1905296974753308No Hit
GTGTCTGGGGAGCACTGTGCGCTCATTGCACAGAGGTAGGTGGCTGAATC46820.18815862551771753No Hit
CAAGTGTCTGGGGAGCACTGTGCGCTCATTGCACAGAGGTAGGTGGCTGA46800.18807825019712046No Hit
ATGTGGTACAGACAGTATTCCAGAAAAGGCCCTGAGTTGCTGATGTACAC46750.1878773118956278No Hit
GAGCACTGTGTGTTCACGGCACAGAGGTAGGTGGCTGAATCACTGGGCTG46290.18602867952189545No Hit
AATGGTGACAAAGAAGATGGAAGGTTTACAGCACAGCTCAATAAAGCCAG45740.1838183582054763No Hit
GCACAGAGGTAGGTGGCTGAATCACTGGGCTGGGAGTCTCTGATGAGCAG45370.18233141477443068No Hit
GTGTTCACGGCACAGAGGTAGGTGGCTGAATCACTGGGCTGGGAGTCTCT44860.18028184409920567No Hit
TTTCAGGGAAGAAGAAGCATGGAAGATTAATTGCCACAATAAACATACAG44790.18000053047711595No Hit
AGCCAGCCAGTATGTTTCTCTGCTCATCAGAGACTCCCAGCCCAGTGATT44550.17903602662995122No Hit
CAGCTCAATAAAGCCAGCCAGTATGTTTCTCTGCTCATCAGAGACTCCCA44470.178714525347563No Hit
CCTTCTTCTGGTACATACAATATTCTGGGAAAAGCCCTGAGTTGATAATG44010.1768658929738306No Hit
ATATACTCCAATGGTGACAAAGAAGATGGAAGGTTTACAGCACAGCTCAA43920.17650420403114384No Hit
GTTTCACTACCCTGCAGTCTAAGCTCTACATCAGGCATTCCTGGGGCATG43180.1735303171690526No Hit
CAGAGCACAGAAGTACACCGCTGAGTCTGACACTTGAACTGAGCCTTTCT42390.17035549200546873No Hit
TTCTCTGCTCATCAGAGACTCCCAGCCCAGTGATTCAGCCACCTACCTCT42370.17027511668487166No Hit
TCTGGGGAGCACTGTGCGCTCATTGCACAGAGGTAGGTGGCTGAATCACT42340.17015455370397609No Hit
ATACTCCAGTGGTAACAAAGAAGATGGAAGGTTTACAGCACAGGTCGATA42160.16943117581860254No Hit
TAATGTCCATATACTCCAATGGTGACAAAGAAGATGGAAGGTTTACAGCA42070.16906948687591578No Hit
AATGTCCATATACTCCAATGGTGACAAAGAAGATGGAAGGTTTACAGCAC42040.16894892389502017No Hit
CAGTATGTTTCTCTGCTCATCAGAGACTCCCAGCCCAGTGATTCAGCCAC41690.16754235578457163No Hit
AAGATGGAAGGTTTACAGCACAGCTCAATAAAGCCAGCCAGTATGTTTCT40980.164689031903376No Hit
GCTCAATAAAGCCAGCCAGTATGTTTCTCTGCTCATCAGAGACTCCCAGC40940.16452828126218189No Hit
CAATATTCTGGGAAAAGCCCTGAGTTGATAATGTCCATATACTCCAATGG40170.16143383141919507No Hit
GGGGAGCACTGTGTGTTCACGGCACAGAGGTAGGTGGCTGAATCACTGGG40020.16083101651471712No Hit
ACTTACAGCAACAGTGCTTTTCAATACTTCATGTGGTACAGACAGTATTC39960.16058989055292594No Hit
CTCTGCTCATCAGAGACTCCCAGCCCAGTGATTCAGCCACCTACCTCTGT39410.15837956923650678No Hit
GAAGATGGAAGGTTTACAGCACAGCTCAATAAAGCCAGCCAGTATGTTTC39150.157334690068745No Hit
TGTACACATACTCCAGTGGTAACAAAGAAGATGGAAGGTTTACAGCACAG38930.15645056154217735No Hit
CCTGTCACTGTGTCACTCAGAGCACAGAAGTACACCGCTGAGTCTGACAC38890.15628981090098323No Hit
CACAGGTCGATAAATCCAGCAAGTATATCTCCTTGTTCATCAGAGACTCA38720.15560662067590822No Hit
GTGCGCTCATTGCACAGAGGTAGGTGGCTGAATCACTGGGCTGTGAGTCT37660.15134672868426405No Hit
GCAGAATTCTGGACCCCTCAGTGTTCCAGAGGGAGCCATTGCCTCTCTCA37590.15106541506217433No Hit
ACTCCAATGGTGACAAAGAAGATGGAAGGTTTACAGCACAGCTCAATAAA37440.15046260015769639No Hit
TGTTCACGGCACAGAGGTAGGTGGCTGAATCACTGGGCTGGGAGTCTCTG37130.14921678268844193No Hit
GCTGATGTACACATACTCCAGTGGTAACAAAGAAGATGGAAGGTTTACAG37110.1491364073678449No Hit
GCCAGTATGTTTCTCTGCTCATCAGAGACTCCCAGCCCAGTGATTCAGCC36790.14785040223829193No Hit
CTCCAATGGTGACAAAGAAGATGGAATGTTTACAGCACAGCTCAATAAAG36480.14660458476903748No Hit
GCGCTCATTGCACAGAGGTAGGTGGCTGAATCACTGGGCTGTGAGTCTCT36360.14612233284545514No Hit
TACAGTGACCGAGGTTCCCAGTCCTTCTTCTGGTACAGACAATATTCTGG36110.14511764133799188No Hit
GGAGCACTGTGCGCTCATTGCACAGAGGTAGGTGGCTGAATCACTGGGCT35950.14447463877321542No Hit
GCAAGGGGAGTTTCACTACCCTGCAGTCTAAGCTCTACATCAGGCATTCC35670.14334938428485655No Hit
CACTTACAGCAACAGTGCTTTTCAATACTTCATGTGGTACAGACAGTATT35570.14294750768187126No Hit
CAATAAAGCCAGCCAGTATGTTTCTCTGCTCATCAGAGACTCCCAGCCCA35490.14262600639948303No Hit
AAGTCCTTCTGTGCTGTCATGCCCTGCTGGGGAGATGAGGCACTCTATAA35020.1407371863654521No Hit
AATGCCCCAACAGCTCTCACTTATTCCTTCACCTACACACCCTGCAGCCA34760.13969230719769035No Hit
ACAATATTCTGGGAAAAGCCCTGAGTTGATAATGTCCATATACTCCAATG34730.13957174421679475No Hit
AGCAACAGTGCTTTTCAATACTTCATGTGGTACAGACAGTATTCCAGAAA34150.13724085991947998No Hit
CGGCACAGAGGTAGGTGGCTGAATCACTGGGCTGGGAGTCTCTGATGAGC34000.13663804501500204No Hit
ATTCCTGGGGCATGGCAGGTTTGGGTACAGACTGCAGGTGTCTGGGGAGC33890.13619598075171824No Hit
GAAGGTTTACAGCACAGGTCGATAAATCCAGCAAGTATATCTCCTTGTTC33310.13386509645440348No Hit
TGCGCTCATTGCACAGAGGTAGGTGGCTGAATCACTGGGCTGTGAGTCTC33260.13366415815291083No Hit
TGGGGAGCACTGTGTGTTCACGGCACAGAGGTAGGTGGCTGAATCACTGT33250.1336239704926123No Hit
CAAAGAAGATGGAAGGTTTACAGCACAGGTCGATAAATCCAGCAAGTATA33200.13342303219111964No Hit
TCCAGTGGTAACAAAGAAGATGGAAGGTTTACAGCACAGGTCGATAAATC33010.1326594666454476No Hit
CACACACACACATCTCCCTAGAAGCATCAATATTTACTGAATTAGAGTAT32110.12904257721857987No Hit
TATATCTCCTTGTTCATCAGAGACTCACAGCCCAGTGATTCAGCCACCTA31670.12727432016544454No Hit
GCTCATCAGAGACTCCCAGCCCAGTGATTCAGCCACCTACCTCTGTGCCG31510.1266313176006681No Hit
CATTGCACAGAGGTAGGTGGCTGAATCACTGGGCTGTGAGTCTCTGATGA31490.12655094228007102No Hit
ACAGGTCGATAAATCCAGCAAGTATATCTCCTTGTTCATCAGAGACTCAC31150.125184561829921No Hit
CCAGTGATTCAGCCACCTACCTCTGTGCAATGAGCGCACAGTGCTCCCCA30520.12265273923111361No Hit
CATGTGGTACAGACAGTATTCCAGAAAAGGCCCTGAGTTGCTGATGTACA30210.12140692176185917No Hit
GGTTTGGGTACAGACTGCAGGTGTCTGGGGAGCACTGTGTGTTCACGGCA30020.12064335621618712No Hit
GTCTAAGCTCTACATCAGGCATTCCTGGGGCATGGCAGGTTTGGGTACAG29830.11987979067051503No Hit
AGTCATGTGGTACAGACAGTATTCCAGAAAAGGCCCTGAGTTGCTGATGT29450.1183526595791709No Hit
AGAGCACAGAAGTACACCGCTGAGTCTGACACTTGAACTGAGCCTTTCTC29170.11722740509081206No Hit
TGGGGAGCACTGTGCGCTCATTGCACAGAGGTAGGTGGCTGAATCACTGG28810.11578064932006497No Hit
AAAGAAGATGGAAGGTTTACAGCACAGGTCGATAAATCCAGCAAGTATAT28650.11513764675528851No Hit
ATACCTGAAACGTCTACATCCACTCTCACCATTCACAATGTAGAGAAACA28290.11369089098454141No Hit
TGATGAGCAGAGAAACATACTGGCTGGCTTTATTGAGCTGTGCTGTAAAC28260.11357032800364583No Hit
CTCATTGCACAGAGGTAGGTGGCTGAATCACTGGGCTGTGAGTCTCTGAT28230.11344976502275024No Hit
GGCAGTAAGGGGTAAAGAAGCAGGTGTCTGGGGAGCACTGTGTGTTCACG27930.11224413521379434No Hit
AAGGCTGATGACAAGGGAAGCAACAAAGGTTTTGAAGCCACATACCGTAA26840.10786368024125458No Hit
GGGAGCACTGTGTGTTCACGGCACAGAGGTAGGTGGCTGAATCACTGGGC26710.10734124065737366No Hit
CATGGCAGGTTTGGGTACAGACTGCAGGTGTCTGGGGAGCACTGTGTGTT26670.10718049001617956No Hit
ACAGCTCAATAAAGCCAGCCAGTATGTTTCTCTGCTCATCAGAGACTCCC26520.1065776751117016No Hit
GGTCACGGCACAGAGGTAGGTGGCTGAATCACTGGGCTGGGAGTCTCTGA26090.1048496057188648No Hit
CACTGTGTGTTCACCACACAGAGGTAGGTGGCTGAATCACTGAGCTTGGA26080.10480941805856628No Hit
CATCAGAGACTCCCAGCCCAGTGATTCAGCCACCTACCTCTGTGCCGTGA26030.10460847975707363No Hit
GTTTGGTACAGACAATATTCTGGGAAAAGCCCTGAGTTGATAATGTTCAT25900.10408604017319274No Hit
GGTGACAAAGAAGATGGAAGGTTTACAGCACAGCTCAATAAAGCCAGCCA25770.10356360058931185No Hit
TGACAAAGAAGATGGAAGGTTTACAGCACAGCTCAATAAAGCCAGCCAGT25670.10316172398632655No Hit
CAGTCCCTGTCACTGTGTCACTCAGAGCACAGAAGTACACCGCTGAGTCT25550.10267947206274419No Hit
ACAGCACAGGTCGATAAATCCAGCAAGTATATCTCCTTGTTCATCAGAGA25380.10199628183766918No Hit
TGTGACAATATTCTGGGAAAAGCCCTGAGTTGATAATGTTCATATACTAC25320.101755155875878No Hit
AGGCTGATGACAAGGGAAGCAACAAAGGTTTTGAAGCCACATACCGTAAG25260.10151402991408683No Hit
AAGGCCCTGAGTTGCTGATGTACACATACTCCAGTGGTAACAAAGAAGAT25250.1014738422537883No Hit
ACTCAGAGCACAGAAGTACACCGCTGAGTCTGACACTTGAACTGAGCCTT25180.10119252863169859No Hit
CAGCCCAGTGATTCAGCCACCTACCTCTGTGCAATGAGCGCACAGTGCTC25100.10087102734931035No Hit
GGTAACAAAGAAGATGGAAGGTTTACAGCACAGGTCGATAAATCCAGCAA25070.10075046436841474No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTCGAA100.00723407144.037751
TCGTGCG700.0144.037731
GCGATCC450.0143.994323
TTCGAAT100.0072405934143.994322
CGTGCGC700.0143.994312
CGCTCTT550.0143.994312
CGAGCCT253.8711732E-8143.991426
CTCGAGC253.8711732E-8143.991424
AGTCGGT500.0143.991425
CGGACCT650.0143.991415
TCGATTC1150.0143.968289
CCTGCTA351.2732926E-11143.968288
GTCGAAG550.0143.968269
TGTCGAT2800.0141.397429
CGTAGAC2450.0141.055652
TCTACCG2150.0140.688021
TGATCGA1250.0138.231776
ATCGGCA5750.0137.733692
ATGGTAC1650.0135.267382
CGTGGGA1350.0133.368271
Produced by FastQC (version 0.11.5)