FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005466937

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005466937
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26423198
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCGTACACATCTCGTAT4222151.5978951525852398TruSeq Adapter, Index 16 (97% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCGTACACATCTCGTA467960.17710195412379684TruSeq Adapter, Index 16 (97% over 37bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT375960.14228406417724304No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG558100.059.63087545
TATGCCG562200.059.14528348
CGTATGC566800.058.659546
CGTACAC568000.058.53397435
CTCGTAT573800.058.01561744
TCGTACA575700.057.8483534
GTATGCC581450.057.1601647
ACTCGTA585750.056.93935832
GCCGTCT587800.056.58170751
CGTCTGA597600.056.34906416
ATGCCGT589950.056.3279349
CACTCGT594850.056.1094831
ACGTCTG606350.055.6051815
TCTCGTA602100.055.36431543
TCACTCG606800.055.25826330
ACACGTC610850.055.2356513
CTCGTAC612000.054.52008433
ATCTCGT612000.054.44002542
CACGTCT621350.054.30787314
GATCGGA589950.053.8346061
Produced by FastQC (version 0.11.5)