FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005466949

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005466949
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25611756
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGCGTGTATCTCGTAT1314430.5132135414690036TruSeq Adapter, Index 27 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG196650.053.8909645
TATGCCG201200.052.66431848
GCGTGTA202200.052.3231736
CGTGTAT202150.052.31879837
CGTATGC205300.051.67603346
TATCTCG208000.050.96541241
CTCGTAT208500.050.9273244
CATGCGT208700.050.8442833
ACATGCG213800.049.7951332
ATGCGTG216000.049.15833734
TCTCGTA225350.047.15032643
GTATGCC225300.047.10619447
CGTCTGA230400.046.5870216
ATCTCGT227850.046.57153742
TGCGTGT228250.046.56604835
ATGCCGT229700.046.23676749
GCCGTCT232250.045.77405551
ACACGTC235200.045.72554413
ACGTCTG235300.045.67637315
GATCGGA229700.044.0661741
Produced by FastQC (version 0.11.5)