FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005468042

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005468042
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6619604
Sequences flagged as poor quality0
Sequence length150
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTGCGA15950.010.8338573
GTGCGAT14501.0913936E-1110.4275874
TGCGATC14601.2732926E-1110.3561655
CGCGATC13252.5356712E-99.7811325
GCGCGAT13352.9747753E-89.1685394
GCGATCT28500.09.0947376
TGGTGCG21003.6379788E-128.5714282
TGGCGCG17102.584784E-98.4210532
ACGATCT17554.2018655E-98.2051296
GGCGCGA15804.4816625E-77.74683573
CACGATC19704.431058E-97.67512665
ATCTCGG52200.07.5862079
GCACGAT19051.9301297E-87.5590554
GATCTCG39750.07.42641548
CGATCTC47850.07.37304077
GGCACGA22204.4112312E-86.8108113
GTGGCGC35600.06.67415761
GAGCCGA40800.06.3529413
CGATCTT15600.00126161316.07
TGGCACG28206.519058E-75.61702162
Produced by FastQC (version 0.11.5)