FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005468078

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005468078
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6503562
Sequences flagged as poor quality0
Sequence length150
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGAT11001.0004442E-1011.7818184
CGCGATC11953.45608E-1111.4476995
TGCGATC14551.2187229E-109.8969075
GGCGCGA13905.5479177E-109.8417263
GCGATCT25000.09.7926
ATCTCGG46100.09.5271159
GGTGCGA15503.976311E-98.8258063
TGGCGCG16451.2478267E-98.7537992
GTGCGAT15302.980778E-88.4705894
GATCTCG36200.07.7569068
AATCTCG19352.5758709E-87.44186078
GTGGCGC34650.07.27272751
CGCAATC12951.3084598E-47.2277995
AGATCGC21602.6131602E-87.09
TGGTGCG21652.7313945E-86.9838342
CGATCTC43550.06.94374287
GCGCAAT12456.569848E-46.93975934
CACGATC19051.1740267E-66.80314975
GCACGAT17351.4853091E-56.6397694
GGCGCAA14254.2252068E-46.56842143
Produced by FastQC (version 0.11.5)