FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005468914

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005468914
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4470730
Sequences flagged as poor quality0
Sequence length150
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCGC14400.016.01
GCGCGAT9851.9826984E-1012.4263962
CGCGCCC14800.012.1621623
CGCGATC9451.7686034E-811.4285723
CCGCGCC20400.011.2941182
GCTCACG38200.010.9319372
CTCACGC38400.010.8753
ACCGCAC14155.456968E-1210.6855131
GGCGCGA11602.9813236E-910.55172351
CCGCACC14651.382432E-109.8293522
CTTCGTT20702.0008883E-118.3478262
CACGCCT51900.08.1849715
ACCGTGC16006.358823E-88.11
CGCACCT10951.4994842E-47.8904113
TCACGCC53250.07.8422534
ACGCCTG50000.07.77600056
GCGATCT19704.42742E-97.67512754
GCGCCCG18801.5106707E-87.65957454
CGTGCAC9600.0028772297.53
CGCCTGT52450.07.1382277
Produced by FastQC (version 0.11.5)