FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005469029

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005469029
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3971429
Sequences flagged as poor quality0
Sequence length150
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGATC6350.019.2755893
GCGCGAT6600.018.5454562
ACCGCGC11650.016.0686681
GGCGCGA8107.2759576E-1215.1111111
CTCACGC37250.012.1771823
GCGATCT14950.012.0401344
GCTCACG38850.011.3050192
ACCGTGC13603.6379788E-1211.1176471
CGCGCCC13252.0008883E-1110.8679253
ACTGCGC13152.0190782E-1010.4030421
CCGTGCC17400.09.9310342
CCGCGCC17353.6379788E-129.5446682
TCACGCC50150.09.1884354
CGCCCGG21200.09.1698115
CACGCCT49500.08.7272735
GCGCCCG16952.1882443E-98.4955754
ACCGCAC11905.0108665E-68.4705881
CGATCTC27750.08.3027035
ACGCCTG47700.08.3018876
CGTGCCC14409.172727E-78.03