FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005469036

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005469036
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3357382
Sequences flagged as poor quality0
Sequence length150
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCGC10200.016.9411771
CTCACGC28550.013.8704033
GCTCACG29150.013.8319032
ACCGTGC12053.6379788E-1211.9502071
CGCGATC7251.1316661E-611.9172423
GCGCGAT7301.2295186E-611.8356172
GGCGCGA8355.076581E-711.2095811
GCGATCT15250.010.8590164
CACGCCT38950.010.7214375
CCGCGCC14155.456968E-1210.6855132
GTGCGAT8204.965832E-610.5365862
TCACGCC39000.010.3384624
ACGCCTG37600.09.9574476
CGCGCCC12005.651964E-89.63
CCGTGCC13704.5038178E-99.4598542
TGCGATC8609.6058604E-59.2093023
GGTGCGA8004.7382756E-49.01
CGCCTGT42700.08.4309137
ACCGCAC9402.5227945E-48.4255311
CGCCCGG18751.6916601E-98.0645
Produced by FastQC (version 0.11.5)