FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005469087

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005469087
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3876170
Sequences flagged as poor quality0
Sequence length150
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCGC10300.018.1747571
GCGCGAT6553.3077413E-713.1908392
CCGCGCC15450.013.0485442
CGCGATC7308.85866E-812.82191853
CGCGCCC12951.8189894E-1211.6756753
GGCGCGA8003.696363E-610.7999991
CTCACGC36850.09.7693353
GCTCACG37750.08.9642382
ACCGTGC13855.3973054E-88.8375451
ACCGCAC10701.2075467E-58.74766351
GCGTGAT11754.223675E-68.5787232
CGCCCGG21003.6379788E-128.5714295
ACTTCGG7800.00376878878.3076931
ACGCCTG48850.08.1064496
GCGCCCG15252.5434383E-78.026234
ATCTCGG27200.07.9411777
CGCCTGT50300.07.8727637
CACGCCT51450.07.83673435
TCACGCC47000.07.81276564
GCGATCT15754.2581996E-77.77142864
Produced by FastQC (version 0.11.5)