FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005469761

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005469761
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25582124
Sequences flagged as poor quality0
Sequence length85
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7582772.9640892992309786No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6187452.4186615622690284No Hit
TTTTTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1822120.7122629848874159No Hit
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1738710.6796581863179149No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC906130.35420436551710877TruSeq Adapter, Index 1 (100% over 50bp)
GCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGA535570.20935321867722947No Hit
CTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGT410620.16051051898583557No Hit
GGAAGCTATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTG402530.15734815451602063No Hit
CTTTGGTATAGTTGGGATTTTAATATCATTAATAGCATGATGGTGATTGT319970.1250756192097263No Hit
GTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGA292740.1144314678484085No Hit
AGGAGATGTTGGAACTCTACCAATTGGAGCTTTCTTAGCTGTCTTAGCAG270060.1055659021901387No Hit
GCCTCACTTAGTTACAGTTTTATGGATAATTGGGATATTCTTTGGTATAG257600.10069531364948431No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG1036450.058.717081
TTTTGGG374700.044.5689162
TCGTATG108850.036.3607542-43
TTTGGGG470850.035.8368233
TATGCCG118800.033.54720746-47
CTCGTAT114700.033.163242-43
TTTTTGG495200.031.5772611
TTGGGGG587150.029.3841174
TCACGAT137400.028.50687634-35
TCTCGTA139800.026.87068640-41
CGTATGC151800.026.34563844-45
GTCCGAT31900.025.6365131
CCGTCTT161350.024.76142550-51
CGATTGG31900.024.6400474
ATCACGA169250.024.35608134-35
ATCTCGT155800.024.09850540-41
GTATGCC167300.023.79852744-45
CGTCTTC171850.023.23701550-51
GCCGTCT173150.022.959948-49
CATCACG183750.022.06879632-33
Produced by FastQC (version 0.11.5)