FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005469763

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005469763
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27619491
Sequences flagged as poor quality0
Sequence length85
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5251221.9012732711113323No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4927641.7841168760133923No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGC2076220.7517227598437639TruSeq Adapter, Index 3 (100% over 50bp)
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1292410.467934039769234No Hit
TTTTTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1095080.39648811775713033No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG984150.042.5524021
TCGTATG241500.037.31654742-43
TATGCCG244250.036.85480546-47
TTTTGGG301000.035.4571652
CTCGTAT250600.035.1024442-43
CGTATGC263050.034.3566144-45
GAGCACA565700.032.4252479
GTATGCC282750.031.96987344-45
ACTTAGG288050.031.5434532-33
TCTCGTA277650.031.37020140-41
AGAGCAC590200.031.1460448
GCCGTCT304700.029.6723448-49
AAGAGCA634850.029.0363757
ATCTCGT301950.028.91103640-41
ATGCCGT322800.028.24142646-47
CCGTCTT322200.028.07284750-51
TTTGGGG386650.027.8273033
TTAGGCA326200.027.8058934-35
CGGAAGA662350.027.4255084
CTTAGGC338800.026.90590132-33
Produced by FastQC (version 0.11.5)