FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005469772

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005469772
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31553204
Sequences flagged as poor quality0
Sequence length85
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3684131.1675929962611722No Hit
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3107930.9849807962449709No Hit
TTTTTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1655330.5246155033891329No Hit
AGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1298190.4114288995817984No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGC977670.3098480902288085TruSeq Adapter, Index 11 (100% over 50bp)
ATTTTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG379060.12013360037858595No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTTGGG444100.040.7784162
TTTGGGG527750.036.851813
TATGCCG142400.034.88208846-47
TCGTATG144750.034.413242-43
CGTATGC153100.033.07701544-45
TTGGGGG664350.032.324014
TTTTTGG476850.029.8002511
ACGGCTA177850.029.08302532-33
CGGCTAC176650.028.84456832-33
CTCGTAT165150.028.04547942-43
GTATGCC186050.027.04912244-45
AGTCACG221050.024.14057528-29
GCGGTAT51400.023.6727561
GCCGTCT208900.023.56012248-49
TCTCGTA192650.023.29433340-41
GCTACAT210250.022.97732234-35
ATGCCGT220500.022.86739546-47
GAGCACA497400.021.9075059
ATCTCGT205050.021.70263140-41
GGCTACA234800.021.56745334-35
Produced by FastQC (version 0.11.5)