FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005469778

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005469778
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36495768
Sequences flagged as poor quality0
Sequence length85
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTAT2653320.7270212809331756TruSeq Adapter, Index 15 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA619050.043.1068342
GATCGGA614650.042.1951261
AGAGCAC640150.041.948138
GAGCACA650800.041.2920769
TCGGAAG649500.041.1638873
CGGAAGA654550.041.0093084
AAGAGCA689100.039.1575787
TCGTATG314050.037.34232744-45
TATGCCG321900.035.8610948-49
GAAGAGC789300.034.3038756
CTCGTAT331100.033.89237644-45
CGTATGC341350.033.79459846-47
GTATGCC370650.032.08223346-47
TCTCGTA368600.031.3980242-43
AATCTCG373500.031.00725740-41
CGTCTGA428000.030.97251716-17
ACGTCTG433700.030.72496614-15
GCCGTCT373050.030.04936850-51
GGAAGAG921600.029.4306685
ACACGTC456400.029.23257412-13
Produced by FastQC (version 0.11.5)