FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005471103

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005471103
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5201678
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAC12750.089.365351
CGAGTTG20150.078.377491
ATACTAG45550.076.943191
CGTGTAC18600.072.502161
CGTGTTG26100.071.838151
ATACTTG64450.071.387151
CGTGTTC28200.070.324371
TGACTAG52550.065.04711
CGGTAGC12450.064.874041
CGAGTAA10250.064.727111
GTAGTAG48950.064.085361
CGGTATG6800.062.5698931
CTACTAG40550.062.4221231
ATACTTC51950.061.49511
ACGTAGG14000.061.2972831
ACTAGGC54300.061.127133
CGTAGCC13850.060.8383642
ACGTAGC12600.060.6677131
CGTACCG2400.060.0150532
CGAGTTC16650.059.770531
Produced by FastQC (version 0.11.5)