FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005471107

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005471107
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12400153
Sequences flagged as poor quality0
Sequence length150
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAC30750.090.253071
CGTGTAC45250.078.218471
CGTGTTC55000.075.0997241
CGAGTAA28700.072.587581
CCGTACG5900.069.641681
CGTAGCC24600.068.797772
CGAGTTG40900.067.679061
CGTGTTG62150.065.184081
CGGTACG5000.064.8767241
CGAGTTC36950.064.379251
ATACTAG114900.063.11381
CGGTACC19300.061.6272851
CGTACGG5750.061.3719332
CGTAGGC29550.060.685292
CGTACCC19350.059.9220282
GCGTACC12300.059.7780081
CCGTTGC29500.059.6230471
CCGTACC17900.059.19851
CGTTGCA33350.059.169282
CCGTACA22750.058.935631
Produced by FastQC (version 0.11.5)