FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005471114

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005471114
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15287434
Sequences flagged as poor quality0
Sequence length150
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGTAG63200.086.347851
CGTGTAC48250.082.622921
CGTGTTC75750.077.942441
CGAGTAC37750.074.6676561
CGAGTTG52350.074.086371
CGTGTTG78000.072.366891
CGAGTAA35000.070.647821
ATACTAG125800.069.740111
ATACTTG182000.066.5442661
CGGTACG6850.063.144191
CGAGTTC51150.062.85821
ATACTTC160500.062.1183471
CGTAGCC32950.059.0154842
CGGTATG22900.058.8679431
CCGTACA27950.058.8065571
CGAGTTA30750.057.9061361
CGGTACA26950.057.7786941
TCGTACC17050.057.502571
CCGTTGC36650.057.4356541
GTAGTAC76400.057.086671
Produced by FastQC (version 0.11.5)