FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005471115

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005471115
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15287434
Sequences flagged as poor quality0
Sequence length150
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAC41150.071.466911
CGTGTTC74650.070.0040051
CGTGTAC50100.067.044461
CGAGTAA33800.066.9618761
CGAGTTG50750.065.6176451
ATACTAG125000.064.756661
ATACTTG179250.061.2026441
CGTGTTG81700.058.2286261
TGACTAG141050.056.6726041
ATACTTC160800.056.5254141
CTACTAG114750.055.5282821
ACTAGGC144100.055.4617163
GTAGTAG133650.053.5004431
CGTAGGC40800.052.4238132
GTAGTAC74250.052.324731
CGGTACT23850.051.6799931
CGAGTTA31350.051.502131
AGTAGGC166400.050.7550163
CGAGTTC49700.050.4704931
CGTAGCC32700.050.2133752
Produced by FastQC (version 0.11.5)