FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005471124

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005471124
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18264060
Sequences flagged as poor quality0
Sequence length150
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGTAG94300.0105.447161
CGTGTAC62900.092.284241
CGTAGGG36850.080.919812
ACGTAGG50850.080.828821
CGAGTAC46300.080.673541
ATACTAG216600.080.363921
CGTGTTG90200.079.0627751
TCGTAGG35550.078.0914461
CCGTACG7300.078.0344851
CGTAGGA39550.076.670382
CGTAGCC31300.075.938432
GCACTAG133650.075.857531
CGGTATG27050.075.7065351
CGGTAGG37700.075.168091
CGAGTTG60450.075.0303041
CTACTAG167000.074.482621
CGAGTAA41000.073.338931
CGTGTTC61400.073.047331
GCGTAGC22650.071.311921
GTAGTAG204800.071.2275851
Produced by FastQC (version 0.11.5)