FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005471125

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005471125
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18264060
Sequences flagged as poor quality0
Sequence length150
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAC45400.075.583561
ATACTAG208600.075.2005161
ACGTAGG55300.074.306351
CGTGTAC69500.072.8162841
GCACTAG130100.072.1456151
CGTAGGC46550.068.842982
ACGTAGC41200.068.240721
TCGTAGG43550.067.869081
CGTAGGG52900.067.5222
CTACTAG160100.067.4522861
CGAGTAA43650.067.383281
TGACTAG203550.066.866271
CCGTAGG42500.066.662231
CGGTAGG42550.066.4144741
CGTGTTC67350.065.828471
CGGTAGC38400.065.519471
GTAGTAG212300.065.434681
CGTAGCC34700.064.543162
TAAGTAG257500.063.6073571
CGAGTTG58600.063.1096761
Produced by FastQC (version 0.11.5)