FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005471126

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005471126
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21530075
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGTAG109000.0106.857641
CGAGTAC44750.094.039421
ATACTAG252200.093.755141
ACGTAGG64400.089.921141
CGTAGGG59600.088.202742
CGTGTAC65900.087.3210141
CGTAGGC56800.087.099152
CGAGTTG60900.085.388521
TCGTAGG47950.085.0257341
CTACTAG216850.082.0312961
CGAGTAA44500.081.12831
CGGTAGC49650.080.057941
CGGTAGG51200.078.346561
TCGTAGC34200.078.051671
GTAGTAG249400.077.934181
ACGTAGC50700.076.6737061
CGTGTTG101400.076.4579241
CGTAGCA45050.076.407962
TGACTAG259700.076.359741
CCGTAGG53350.075.4626161
Produced by FastQC (version 0.11.5)