FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005471127

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005471127
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21530075
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAC45550.091.157961
ATACTAG253000.091.040891
CGTGTAC70600.084.930231
ACGTAGG70600.081.948421
TCGTAGG50050.081.946671
CGTAGGC55200.080.48782
GTAGTAG256000.080.21951
CTACTAG208500.079.380851
TGACTAG252950.078.546551
CGTAGGG67050.077.539392
CGGTAGG56050.077.0598751
CGAGTAA47200.076.897991
CGAGTTG64900.076.394661
TAAGTAG300800.075.101521
CGTAGGA54750.074.178662
GCACTAG179450.074.1086651
CCGTAGG50050.073.244371
TAGTAGG323650.070.7292252
TATGTAG281900.070.531631
CGTAGCA50350.069.648772
Produced by FastQC (version 0.11.5)