FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005471152

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005471152
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13316869
Sequences flagged as poor quality0
Sequence length150
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGTAG67450.0107.454491
CGTGTAC44900.092.837191
ATACTAG152400.089.011131
CGAGTAC30200.084.773841
CGTAGGC40900.084.358152
CGTAGGG35550.081.0466842
ACGTAGG42200.079.9786151
TCGTAGG31050.079.665991
CGAGTTG43700.078.553581
CCGTACG6250.076.1560441
CGGTAGG32350.075.79581
CGTAGCC33600.075.460252
CGGTAGC29350.074.943191
CTACTAG133000.074.828651
CGTGTTG68600.074.6405261
GTAGTAG152400.074.0103151
CGTACGG7350.073.5002442
TGACTAG156700.072.117471
ATACTTG180400.071.55781
TAAGTAG184250.071.080231
Produced by FastQC (version 0.11.5)