FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005471153

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005471153
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13316869
Sequences flagged as poor quality0
Sequence length150
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACTAG143750.086.713051
CGTGTAC48400.083.7121051
CGTAGGC39100.078.643092
CGAGTAC31450.076.563741
CGAGTTG42850.076.2157441
ACGTAGG46850.075.8638151
CGTACGG9250.075.5160752
CGTAGCC33100.073.753242
GTAGTAG153200.072.846641
CTACTAG120150.071.763691
TGACTAG159500.071.460911
TAAGTAG178150.070.818961
TATGTAG166050.069.8577351
ACTAGGC169100.069.444043
ATACTTG167050.069.180611
TCGTAGG32050.068.607111
ACGTACC24300.068.236851
CGTAGGG45700.068.2308962
GCACTAG105950.068.1811141
CGGTAGG36800.068.175571
Produced by FastQC (version 0.11.5)