FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005471158

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005471158
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10861314
Sequences flagged as poor quality0
Sequence length150
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGTAG56850.094.50221
CGTGTAC35650.093.0495761
CGTAGGG26000.092.530932
ATACTAG114500.083.638981
ACGTAGG32900.078.689121
CGGTACG5250.076.920991
TCGTAGG24450.076.390081
CGGTATG14600.076.064841
ACGTAGC22300.075.9939651
CGTAGCC20900.075.131832
GCACTAG74600.074.723421
CGTGTTC44500.074.058051
CGGTAGC21200.073.474051
CGAGTAA22100.073.418641
GTAGTAG112100.073.014041
CTACTAG95150.072.909211
TAAGTAG127050.072.766161
TCGTAGC16800.071.254941
ATACTTG134650.070.640631
CGGTAGG26000.070.449271
Produced by FastQC (version 0.11.5)