FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005471159

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005471159
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10861314
Sequences flagged as poor quality0
Sequence length150
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACTAG110100.082.2466351
CGTGTAC44000.076.520891
ACGTAGG30450.075.0564041
GCACTAG74950.073.683961
CGGTAGC21850.073.5816041
CGTAGGG29050.071.643252
ATACTTG130550.070.85421
CGTAGCC19350.070.71252
TAAGTAG132750.070.223061
GTAGTAG114800.069.647491
CGGTATG16700.068.643031
CTACTAG87450.068.510481
CGTACCC16000.068.4143452
CGTGTTC44300.068.35361
TCGTAGG23850.068.3181151
ACGTAGC23800.067.249921
TGACTAG113350.066.022451
TAGTAGG130250.065.68442
CGTACGC5000.064.813592
CGGTAGG26650.064.657141
Produced by FastQC (version 0.11.5)