FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005471168

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005471168
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23579859
Sequences flagged as poor quality0
Sequence length150
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGTAG121350.0106.824461
CGTGTAC75600.093.034761
CGAGTAC51650.092.179861
CGTAGGG54700.085.0710142
CGTGTTG113500.081.989131
ATACTAG269900.081.679471
CGTAGGC62450.081.319192
CGAGTTG77450.080.101281
CGGTAGG52600.079.954931
ACGTAGG74950.079.596931
CGGTAGC46450.079.359261
CGTAGCC49000.077.0320742
TCGTAGG51750.075.552941
CGTGTTC91550.075.3289641
CCGTAGG52100.075.1838461
CTACTAG236050.074.720081
CGAGTAA48550.074.1436161
TCGTAGC38600.074.006581
ACGTAGC55800.073.947631
GTAGTAG265750.072.6127551
Produced by FastQC (version 0.11.5)