FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005471172

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005471172
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24651759
Sequences flagged as poor quality0
Sequence length150
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGTAG117400.0101.870461
CGTGTAC85500.093.477151
CGAGTAC55850.087.0499651
CGTAGGG48000.086.2901842
CGTGTTG121950.084.051231
CGGTAGG49350.081.560311
CGAGTTG79100.081.0694351
ACGTAGG70650.079.6368941
ATACTAG261000.079.567011
CGTGTTC89100.078.6901551
CGGTACG10050.077.515681
CGAGTAA48650.076.803111
CGTAGGC63500.074.982952
CGGTAGC47150.074.503921
TCGTAGG50950.073.7607651
CTACTAG223650.071.084441
CGTAGCC44950.069.870142
CCGTAGG51100.069.4506151
CGGTATG32650.069.150131
GCACTAG183400.069.025511
Produced by FastQC (version 0.11.5)