FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005471176

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005471176
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23733826
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGTAG125750.0104.676311
CGTGTAC80300.092.156351
CGTAGGG68200.089.5647352
ATACTAG277250.088.6067051
CGAGTAC53300.086.470341
CGTAGGC69850.085.0771942
ACGTAGG83100.083.496371
CGAGTTG77950.081.517951
TCGTAGG57850.081.040761
CGGTACG9000.080.1403051
CGGTAGG56950.079.7885061
CGTGTTG122050.077.888111
CGTAGCC57350.077.746862
CTACTAG234300.077.1748051
CCGTAGG58800.075.6835251
TCGTAGC44300.074.242841
GTAGTAG283950.074.145651
CGTACGG13450.072.835362
TAAGTAG336050.072.780891
ATACTTG312050.072.1147161
Produced by FastQC (version 0.11.5)