FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005471182

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005471182
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18560013
Sequences flagged as poor quality0
Sequence length150
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGTAG100500.097.3839341
CGTGTAC58350.094.6806951
ATACTAG187550.084.793991
CGTAGGG38150.083.834812
CGGTACG8700.078.754951
ACGTAGC36850.078.092441
CGGTAGC33300.077.754181
CGGTATG26050.077.2449341
CGGTAGG39050.076.832661
CGTGTTC73950.076.755611
CGTAGCC30350.076.187242
CTACTAG154000.075.9164351
GTAGTAG192650.075.511031
GCACTAG130200.075.44661
ACGTAGG51500.075.343991
ATACTTG228700.074.046661
TCGTAGG41000.073.706151
CGAGTAA39850.073.299371
CGGTACA26950.072.524391
TAAGTAG220300.071.664621
Produced by FastQC (version 0.11.5)