FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005471183

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005471183
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18560013
Sequences flagged as poor quality0
Sequence length150
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACTAG185700.081.496661
GCACTAG129950.077.232881
CGTGTAC66600.075.9981
ACGTAGG54350.075.085521
CGGTAGC35250.073.8392641
CTACTAG153600.073.415011
CGGTACG9150.073.2826461
GTAGTAG196100.072.17421
CGGTAGG44550.071.696071
ATACTTG221200.071.318381
CGTAGCC34550.070.451522
CGGTATG27600.070.2720261
TAAGTAG216950.070.123261
CGTAGGG49800.069.7010652
TGACTAG193500.069.343321
CGTGTTC72700.069.3237461
TAGTAGG222900.067.103962
ACTAGCG29800.066.9253163
CGAGTAA39150.066.667791
CGTAGGA43600.066.564132
Produced by FastQC (version 0.11.5)