FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005471186

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005471186
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23286575
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACTAG225750.0105.020521
CGTGTAG142150.0104.119651
CGTGTAC81050.092.996261
ATACTTG262350.089.379551
CGTAGGG64550.088.827742
TAAGTAG275300.088.109531
TATGTAG242500.088.01071
CTACTAG211400.085.5023961
ACGTAGG81550.083.139251
GTAGTAG256050.082.592651
CGTACGG20250.082.526762
CGTAGCC63150.082.4701462
TGACTAG265550.077.8452451
TCGTAGC42750.077.6111151
TCGTAGG57400.077.405361
TAGTAGG315200.077.3578342
CGAGTAC63750.077.162511
CGGTAGG62500.076.5130841
CGTAGGC85850.076.3541952
ACGTAGC62700.075.0036241
Produced by FastQC (version 0.11.5)