FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005471192

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005471192
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5117299
Sequences flagged as poor quality0
Sequence length150
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGTAG26150.0107.018181
CGTGTAC19150.093.783871
CGTAGGG11250.088.9948962
CGGTAGC9500.086.552331
CGTACGG2600.085.879822
CGAGTAC11400.085.413491
CGTAGCC10800.079.3644562
CGTGTTG26100.079.035661
ATACTAG58650.078.3373641
CGTGTTC18500.076.415571
TCGTAGG10600.075.529161
GTAGTAG59050.075.241651
CGGTAGG10850.074.453621
ACGTAGC14700.074.0895841
ACGTAGG15500.073.988281
CGAGTAA10300.073.5274661
CGTAGAC8800.072.846742
CTACTAG49250.072.200171
TCGTACG1700.072.1269451
CGAGTTG15900.071.673321
Produced by FastQC (version 0.11.5)