FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005471193

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005471193
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5117299
Sequences flagged as poor quality0
Sequence length150
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAC12750.083.12651
ATACTAG59600.079.236891
CGTAGGC13550.076.522042
CGTGTAC18250.075.8526461
CGTGTTG27100.073.695821
ACGTAGG15100.073.054481
TCGTAGG12850.072.941151
CGGTAGG12800.071.536241
GTAGTAG59100.071.3675461
CGAGTAA11150.071.129571
CGAGTTG18450.070.340991
CGGTACG2500.069.215541
CGTAGGG15250.068.4638752
CGTACCC7900.068.359282
CGGTAGC10200.067.858371
CGCTAGC6700.067.795071
GCACTAG41400.067.745691
CGTGTTC19950.067.5821
ATACTTG67700.066.881091
CCGTAGG12750.066.7273941
Produced by FastQC (version 0.11.5)