FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005471196

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005471196
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16611809
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGTAG86900.0102.902141
CGTGTAC55450.095.198911
CGAGTAC42700.083.936521
CGTAGGG39250.082.950712
ATACTAG183100.082.866661
CGGTAGC29850.078.999791
CGTGTTG81900.078.718371
ACGTAGG51500.077.575581
CGGTAGG36550.074.7780761
CGAGTAA34050.074.7618561
TCGTAGG36200.074.704221
CGAGTTG54800.074.614791
CTACTAG153900.072.770491
CGGTACG6050.071.518491
CGTAGGC47900.071.279412
CGGTATG23400.070.881751
GCACTAG125100.070.558051
CGTAGCC34200.070.5569842
CGTAGCA36500.070.255192
CGTGTTC64650.069.716241
Produced by FastQC (version 0.11.5)