FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005471211

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005471211
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19125940
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACTAG188200.095.766451
ATACTTG210750.081.790911
TAAGTAG231300.080.82021
TATGTAG206050.078.548291
CTACTAG170700.077.11881
TGACTAG213600.077.0881961
CGTGTAC75450.076.4405441
ACGTAGG68150.075.213661
GTAGTAG209100.074.747771
TAGTAGG246800.072.714872
CGAGTAC49000.072.534371
CGTAGGC69200.071.91052
ACGTAGC50900.071.526441
ACTAGGC268750.071.369553
CGTAGCC53450.071.004072
AGTAGGC292250.070.730363
TCGTAGG46300.070.5359041
GCACTAG154800.070.4629751
CGGTAGC47750.069.148871
CGTACCC36850.068.985462
Produced by FastQC (version 0.11.5)