FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005471214

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005471214
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7612297
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGTAG33000.098.115481
CGTGTAC23850.088.5899351
CGTAGGG18950.079.829092
CGAGTAC17200.079.6581651
TCGTAGG14950.078.623351
CGTGTTG43400.078.259361
CGTGTTC31600.078.044841
CGAGTTG30250.077.236891
ATACTAG70600.075.9929661
CTACTAG63900.073.127261
ACGTAGG22800.072.427881
CGTAGCC18950.071.846182
CGGTAGC16650.070.595741
GCACTAG54200.069.84981
ACGTTGC19450.068.96021
GTAGTAG73950.068.844881
ATACTTG99500.068.415431
CGTACCC12400.067.388752
CGGTATG10200.066.4557951
CGAGTAA14150.066.250941
Produced by FastQC (version 0.11.5)