FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005471215

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005471215
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7612297
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGTAC24750.075.439531
CGTGTTG44500.073.87191
CGAGTAC19050.073.7928161
CGAGTTG27500.073.4006351
ACGTAGG23200.071.7791751
ATACTAG71000.070.871481
CTACTAG61700.070.6878361
GCACTAG56600.068.268891
ATACTTG97700.068.105411
CGTGTTC33600.067.7988361
TGACTAG79450.066.69111
CGGTATG9850.065.868951
GTAGTAG71850.064.916031
CGTAGCC18400.064.578312
CGTAGGC20850.064.243242
TCGTAGG18300.064.211231
CGGTAGC18350.064.036261
ACGTAGC18600.062.40041
CGTAGGG26150.061.962852
ACTAGGC79850.061.7649463
Produced by FastQC (version 0.11.5)