FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005471218

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005471218
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19133756
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGTAG83950.0100.778911
CGTAGGG48300.088.290422
CGTGTAC61050.088.016261
CGTGTTG103200.087.292131
CGAGTAC39100.087.2522661
CGAGTTG66550.086.161131
TCGTAGG41650.080.178571
ATACTAG180150.078.15161
ACGTAGG61050.078.092281
CGTAGGC51750.077.532512
CGTGTTC77650.077.3741461
CGGTAGC37350.076.664131
CGGTAGG42850.075.576691
CGTAGCC41350.075.431272
CGGTTGC37550.073.7587661
CCGTTGC51050.073.609571
CGGTACG7350.073.5980451
CGTACGG10200.072.740462
CCGTACG9400.072.126081
GCGTAGG32150.070.89221
Produced by FastQC (version 0.11.5)