FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005471219

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005471219
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19133756
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTTG68150.083.579291
CGAGTAC41650.082.400411
CGTGTTG104150.075.1809841
CGGTAGC39850.073.819191
ATACTAG178200.073.556941
CGTGTAC62000.072.332951
CGGTACG9500.072.1003651
CGTAGCC43350.071.931552
CGTAGGC52800.070.241522
CGTGTTC78700.070.176471
ACGTAGG59200.069.6645361
CGGTAGG47300.068.746861
CGTAGGG60000.068.5339052
CTACTAG162500.068.151481
CGAGTAA36950.067.5148241
ATACTTG252600.067.50491
GTAGTAG191650.066.476041
TCGTAGG45000.065.851661
GCACTAG149350.065.2692951
CGTTGCA49100.064.534482
Produced by FastQC (version 0.11.5)