FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005471224

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005471224
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19523736
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGTAG85800.095.490361
CGAGTTG70300.086.895351
ATACTAG179900.082.465361
CGAGTAC42400.079.946681
CGTGTAC61700.079.720061
ACGTAGG61000.078.7431641
CTACTAG166500.078.4463351
CGTAGGG52850.078.232552
CGTGTTG102750.076.438911
CGTAGGC55600.075.917782
ATACTTG259700.075.649071
ACGTAGC45500.074.658281
CGTAGCC48100.073.828242
CGGTAGC44300.071.959311
TCGTAGG43900.071.793541
GTAGTAG194400.070.267121
GCACTAG148400.070.080921
TGACTAG203300.067.403851
ACTAGGC241000.066.804083
CGGTTGC46100.066.646471
Produced by FastQC (version 0.11.5)