FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005471225

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005471225
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19523736
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACTAG179550.080.8693161
CGAGTTG67700.080.828181
CGAGTAC41450.076.357151
ATACTTG249100.075.105781
ACGTAGG65600.074.29381
CGTAGGC58400.072.13672
CTACTAG165000.071.04711
GCACTAG152250.069.7517551
CGTGTAC69600.069.091771
GTAGTAG202600.068.60841
CGTGTTG110800.067.671111
CGGTAGC47350.067.147261
TGACTAG207050.067.133841
TCGTAGG44500.065.939261
CGTAGCC48950.065.760952
CGTGTTC82250.065.127231
ACTAGGC238250.065.124143
TAGTAGG245800.064.894082
TAAGTAG231000.064.4804461
TATGTAG210100.063.8258481
Produced by FastQC (version 0.11.5)