FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005471226

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005471226
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18792595
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGTAG83850.092.1462251
ATACTAG160700.087.9479141
CTACTAG147150.082.669061
CGTGTAC63200.082.474881
ATACTTG214200.080.60961
GTAGTAG188200.076.4287951
GCACTAG130950.076.1185461
ACGTAGG63100.073.725781
CGTGTTG97800.073.499621
TGACTAG171600.073.382061
TAGTAGG235100.071.680472
TAAGTAG202700.071.252491
GTAGTAC81950.070.711971
TCACTAG167200.070.462791
ACTAGCG67500.070.096963
ACGTAGC46700.070.094741
ACTAGGC231400.068.407223
CGAGTAC49950.068.0432051
TATGTAG182900.067.7599951
TACTAGG186800.067.622362
Produced by FastQC (version 0.11.5)