FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005471227

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005471227
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18792595
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACTAG157500.087.964561
CTACTAG145850.081.64381
ATACTTG214600.078.83351
GTAGTAG183300.075.543221
TGACTAG179400.073.500221
GCACTAG133350.072.8808441
CGTGTAC64950.071.5930941
TAGTAGG228950.069.84392
GTAGTAC81700.069.323251
TAAGTAG198450.068.924741
ACGTAGG62150.068.67231
TCACTAG170450.068.6108251
ACTAGGC222450.067.9294053
TACTAGG179050.067.675322
ACTAGCG70700.066.797813
AGTAGGC247900.066.4732743
CGAGTTG78850.066.239111
CGTGTTG108150.066.157491
TCGTAGG47900.065.944651
CGAGTAC48900.065.497431
Produced by FastQC (version 0.11.5)