FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005471232

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005471232
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13174829
Sequences flagged as poor quality0
Sequence length150
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGTAC35900.091.4010541
CGTGTAG60350.090.578731
CGTGTTC55850.088.321651
CGGTACG5750.087.793921
CGTAGGG26150.081.256222
ATACTTG157150.079.06881
ACGTTGC31350.078.902511
CTACTAG95950.078.617811
CGTACCC17300.076.608692
CGGTTGC21350.076.338711
CGGTAGC22900.076.210381
ACGTACG9050.075.702341
GCACTAG80600.075.158571
ATACTAG108600.074.5070341
GTAGTAG113950.073.034111
GCGTACC9550.071.738861
CGTAGCC23850.071.5758062
TCGTAGG27700.070.2941
ACGTAGG37300.070.183021
CGGTATG16850.069.3344961
Produced by FastQC (version 0.11.5)