FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005471233

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005471233
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13174829
Sequences flagged as poor quality0
Sequence length150
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGTTC57500.084.134261
CGGTACG6750.080.107961
CGTGTAC40200.077.4775541
ATACTTG150650.074.944691
ATACTAG107850.074.6708761
CGTAGCC24450.073.926482
GCACTAG79650.072.595021
ACGTAGC25550.071.3917161
ACGTTGC31050.071.284481
CGGTAGC24400.071.210731
CGTACCT18800.070.862922
CGTACGG11550.070.4533542
CTACTAG95150.070.392291
GTAGTAG114950.069.431551
CGGTATG19050.069.25871
CGTACCC17500.069.131582
GTAGTAC59150.067.282571
TCACTAG106600.066.415961
CGTTGCC29950.066.1212542
CCGTACG10050.065.99941
Produced by FastQC (version 0.11.5)