FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005471236

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005471236
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6500656
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACTAG50250.092.432961
CGTGTAG29200.089.660441
ACGTACG3900.088.767441
ATACTTG75200.088.1403351
CGTACGG6650.086.645662
CGTGTAC21700.085.085841
CGGTACG3650.084.967471
CGTAGGG17100.082.554062
CGTAGCC16500.079.881392
TCGTAGG13900.078.8689041
CGTGTTC28850.078.7484051
ACGTAGG21100.077.592631
CTACTAG54550.076.949411
CGTAGGC23600.076.602012
GTAGTAG59950.076.514711
CGTACCC13750.075.95282
GTAGTAC28950.075.735951
TCGTACG4000.075.729721
TATGTAG58050.074.7947851
TAAGTAG67900.074.460391
Produced by FastQC (version 0.11.5)