FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005471237

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005471237
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6500656
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACTAG50400.090.698321
ATACTTG72650.084.8537451
CGTAGCC17150.077.678332
CGAGTAC17600.074.968521
CGTACCC15750.073.610132
GTAGTAG61250.073.219171
CTACTAG53400.073.046011
ACGTAGG22450.072.903781
CGTACGG6350.072.576922
ACGTACC12950.072.379261
ATACTTC54200.071.967851
CGAGTTG27900.071.8424451
CGTAGGC25300.071.725282
TAAGTAG64700.071.2093661
CGTGTAC27650.070.4059141
ACTAGGC87150.070.140543
GTAGTAC26650.069.801221
TCGTAGG16600.069.060471
TAGTAGG72550.068.287832
TAAGTTG58250.068.201851
Produced by FastQC (version 0.11.5)