FastQCFastQC Report
Wed 15 May 2024
EGAF00005471238

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005471238
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences211641970
Total Bases31.7 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAA15941370.7532234745310677No Hit
CGAGCGACCGGCAAGGCGGAGGTCGACCCACGCCACACGTCGCACGAACG11600730.5481299384994385No Hit
GCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAAC5428760.2565067788775544No Hit
GCGACCGGCAAGGCGGAGGTCGACCCACGCCACACGTCGCACGAACGCCT4748610.2243699583782933No Hit
TTCCGGACCCGCCTTCCTCCTCCCCCACCACCACACCGCACGCAACACGC4023320.19010029059926062No Hit
GGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAG3764430.17786783972952058No Hit
GGAAGAAGAGGATCGGCGGGCGGCGGGCGGGGAAGAGGGCACAGACGGGC3713230.17544865982867197No Hit
GGGAAGAAGAGGATCGGCGGGCGGCGGGCGGGGAAGAGGGCACAGACGGG3328020.15724763854730703No Hit
CCGGACCCGCCTTCCTCCTCCCCCACCACCACACCGCACGCAACACGCCC3249600.15354232433198386No Hit
CTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACG2728090.12890118155675834No Hit
CTTCCCCTTCCGGACCCGCCTTCCTCCTCCCCCACCACCACACCGCACGC2659020.12563765117098466No Hit
CGACCGGCAAGGCGGAGGTCGACCCACGCCACACGTCGCACGAACGCCTG2474990.11694230591408689No Hit
TCCGGACCCGCCTTCCTCCTCCCCCACCACCACACCGCACGCAACACGCC2451890.1158508399822587No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG2410180.11388005885600101No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGCTTGTATCTCGTAT2364350.11171460934709689TruSeq Adapter, Index 10 (97% over 39bp)
GCGAGCGACCGGCAAGGCGGAGGTCGACCCACGCCACACGTCGCACGAAC2117580.10005482371951084No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)