FastQCFastQC Report
Tue 28 Jun 2022
EGAF00005471239

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005471239
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences211641970
Sequences flagged as poor quality0
Sequence length150
%GC60

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGA9131740.4314711302299823No Hit
GGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGA4608940.21777060570736514No Hit
CCTCCCTCGGGGGGCTCCCTCGCGCCGCGCGCGGCTCGGGGTTCGGGGTT4343580.2052324498774983No Hit
GGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCG4322590.20424068061736528No Hit
GTTCCTCCCTCGGGGGGCTCCCTCGCGCCGCGCGCGGCTCGGGGTTCGGG3792050.17917287388697054No Hit
CCGGGTTCCTCCCTCGGGGGGCTCCCTCGCGCCGCGCGCGGCTCGGGGTT3177030.15011342032017563No Hit
CGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTG3139110.14832171520610962No Hit
CGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGA3130980.14793757589763507No Hit
CCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCCAGGAGTT3112950.14708566547551982No Hit
GCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGC3093020.14614398079927152No Hit
GCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTT2892610.13667468697253196No Hit
CGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCT2763160.13055822528962474No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCCTCGAATGTGTAGATCT2398550.11333054592149185Illumina Single End PCR Primer 1 (96% over 32bp)
GTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGAT2213950.10460826838835416No Hit
GGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCG2174390.10273907391808912No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)